ID
7257

Mammalian Cell Culture

Introduction to techniques for culturing and maintaining mammalian cells, including proper use of equipment, aseptic technique, media formulation, storage, counting and subculturing of cells. Contents include proper growth conditions, cell cycle regulation, cellular responses to DNA damage, growth patterns, viability assays, transfection and an introduction to the use of fluorescent molecules in visualization of cellular structures.

Stem Cell Technology

A general introduction to the principles of stem cell biology. Topics include embryonic stem cells in early development, adult stem cells, and potential applications of stem cell culture and ethical issues involved in stem cell research. Current research methods involving cell differentiation and fluorescent technology will be presented. Emphasis on laboratory techniques including culture of mouse embryonic stem cells, analysis of stem cells by immunofluorescence and flow cytometry.

Mol and Cell Biotechnology

Introduction to the principles and techniques of molecular and cell biology and protein analysis. Emphasis on lab techniques, such as spectroscopy, preparation of cell lysates, Western blot, immunoprecipitation, enzyme assays, analysis of signal transduction, and stable transfection of mammalian cells. Design and analysis of molecular and cell biology experiments is taught.

GLP and GMP Principles

A general overview of Food and Drug Administration regulations as they pertain to the biotechnology field. Knowledge of Current Good Laboratory Practices (cGLP) and Current Good Manufacturing Practices (cGMP) is needed to work in biotechnology manufacturing and preclinical research laboratories. The course will emphasize those practices as they pertain to the biopharmaceutical industry.

Engineering Design Graphics

Principles of visually communicating engineering designs. Topics include technical sketching, technical drawing, engineering graphics and design; 3-D visualization with orthographic projection; dimensioning and tolerancing practices; computer aided drafting/design (CAD) software; graphical analytical methods of solutions to three-dimensional problems. Assignments develop sketching and 2-D and 3-D CAD skills. The use of CAD software is an integral part of this course.

Intro to Environmental Science

An examination of the scientific evidence informing our understanding of the causes and consequences of human impacts on the environment. Application of core principles, methods, qualitative and quantitative reasoning from the natural sciences, social sciences, and engineering and technology to investigate and evaluate sustainable solutions to environmental degradation and resource depletion.